Discriminative and quantitative analysis of norepinephrine and epinephrine by Surface Enhanced Raman Spectroscopy with gold nanoparticle suspensions - Lipides : systèmes analytiques et biologiques
Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2021

Discriminative and quantitative analysis of norepinephrine and epinephrine by Surface Enhanced Raman Spectroscopy with gold nanoparticle suspensions

Résumé

Surface enhanced Raman spectroscopy (SERS) is a powerful analytical technique capable of increasing the Raman signal of an analyte using specific nanostructures. The close contact between those nanostructures, usually suspension of nanoparticles, and the molecule of interest produces an important exaltation of the intensity of the Raman signal. Even if the exaltation leads to an improvement of Raman spectroscopy sensitivity, the complexity of SERS signal and the numbers of parameters to be controlled conduct to use SERS for detection rather than quantification. The aim of this study was to develop a robust discriminative and quantitative analysis in accordance with pharmaceutical standards. In this present work, we develop a discriminative and quantitative analysis based on the previews optimized parameters by design of experiments fixed for norepinephrine (NOR) and extended to epinephrine (EPI) which are two very close structure neurotransmitters. Studying the short evolution of the Raman signal intensity over time coupled with chemometric tools allowed to identify the outliers and to remove them from the data set. The discriminant analysis showed an excellent separation of EPI and NOR. The comparative analysis of the data showed the superiority of the multivariate analysis after logarithmic transformation. The quantitative analysis allowed the development of robust quantification models from several gold nanoparticle batches with limits of quantification obtained were 32 µg/mL for NOR and below 20 µg/mL for EPI even though no Raman signal is observable for such concentrations. This study improves SERS analysis over ultrasensitive detection for discrimination and quantification using handled Raman spectrometer.
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Dates et versions

hal-04664781 , version 1 (30-07-2024)

Identifiants

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Antoine Dowek, Marion Berge, Patrice Prognon, François-Xavier Legrand, Eric Larquet, et al.. Discriminative and quantitative analysis of norepinephrine and epinephrine by Surface Enhanced Raman Spectroscopy with gold nanoparticle suspensions. Analytical and Bioanalytical Chemistry, 2021, 414 (2), pp.1163-1176. ⟨10.1007/s00216-021-03743-4⟩. ⟨hal-04664781⟩
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